HPLC Analysis of Hemoglobin Variants on Discovery^® BIO PolyMA-WAX

column

Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)

mobile phase

[A] 10 mM Tris, pH 8.0 with acetic acid; [B] 10 mM Tris, 0.25 M KCl, pH 8.0 with acetic acid

gradient

0 to 32% B in 20 min. (linear)

flow rate

0.5 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

50 μg each Hb variants (A2, S and A0)