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  • [Establishment of in vitro culture, plant regeneration and genetic transformation of Camelina sativa].

[Establishment of in vitro culture, plant regeneration and genetic transformation of Camelina sativa].

TSitologiia i genetika (2013-07-05)
A I Emets, Iu N Boĭchuk, E N Shisha, D B Rakhmetov, Ia B Blium
PMID23821950
摘要

The results on in vitro culture establishment, plantlet regeneration and rooting of Camelina sativa cultivar sample Peremozhets and cultivar Mirazh are presented. Effective concentrations of sterilizing agents and duration of plant material treatment were estimated. Phytohormone ratio, sucrose concentration in nutrient medium that induce effective formation of C. sativa shoots and NAA concentration for plantlet rooting have been established. The method of Agrobacterium-mediated transformation of Camelina by using binary vector pGH217 carrying reporter beta-glucoronidase (gus) gene driven under 35S CaMV promoter and nos-terminator, and selective marker hpt gene conferring hygromycin-resistance in transgenic plant was elaborated.

材料
货号
品牌
产品描述
G7017
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于罗曼蜗牛, Type HP-2, aqueous solution, ≥100,000 units/mL
H3274
Sigma-Aldrich
潮霉素B 来源于吸水链霉菌, powder, BioReagent, suitable for cell culture, suitable for insect cell culture
G0751
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于罗曼蜗牛, Type H-1, partially purified powder, ≥300,000 units/g solid
G0876
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于罗曼蜗牛, Type H-2, aqueous solution, ≥85,000 units/mL
G7396
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于大肠杆菌, Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
G1512
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于罗曼蜗牛, Type H-5, lyophilized powder, ≥400,000 units/g solid
G8132
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于帽贝 (Patella vulgata), Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid
G7646
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于大肠杆菌, Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
G7770
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于罗曼蜗牛, Type HP-2S, aqueous solution, ≥90,000 units/mL
H7772
Sigma-Aldrich
潮霉素B 来源于吸水链霉菌, lyophilized powder
H0654
Sigma-Aldrich
潮霉素 B 溶液 来源于吸水链霉菌, ≥60% (HPLC), 45-60 mg/mL in H2O
H9773
Sigma-Aldrich
潮霉素B 来源于吸水链霉菌, suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder
G0251
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于牛肝脏, Type B-1, ≥1,000,000 units/g solid
G8885
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于罗曼蜗牛, Type H-3, aqueous solution, ≥90,000 units/mL
G0799
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于大肠杆菌, >20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution
G8420
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于大肠杆菌, ≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder
G8295
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于大肠杆菌, ≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder
G8162
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于大肠杆菌, aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret)
G0376
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于牛肝脏, Type B-3, ≥2,000,000 units/g solid
H5527
Sigma-Aldrich
潮霉素 B 溶液 来源于吸水链霉菌, ≥60% (HPLC), 45-60 mg/mL in H2O, γ-irradiated
G0762
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于罗曼蜗牛, Type H-3AF, aqueous solution, ≥60,000 units/mL
G4259
Sigma-Aldrich
β-葡萄糖醛酸酶 来源于散大蜗牛 （花园蜗牛）, Type HA-4