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Merck

581615-U

Supelco

Ascentis® Phenyl (5 µm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

别名:

Phenyl Bonded Phase HPLC Column

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Phenyl 高效液相色谱柱, 5 μm particle size, L × I.D. 5 cm × 4.6 mm

材料

stainless steel column

agency

suitable for USP L11

產品線

Ascentis®

特點

endcapped

製造商/商標名

Ascentis®

包裝

1 ea of

標籤範圍

19% Carbon loading

參數

≤70 °C temp. range
400 bar pressure (5801 psi)

技術

HPLC: suitable
LC/MS: suitable

長度 × 內徑

5 cm × 4.6 mm

表面積

450 m2/g

表面覆盖率

5.2 μmol/m2

雜質

<5 ppm metals

基質

fully porous particle
silica gel high purity, spherical

基質活性組

phenyl phase

粒徑

5 μm

孔徑

100 Å

工作pH值範圍

2-8

應用

food and beverages

分離技術

reversed phase

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相关类别

一般說明

Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质,孔径 100Å,硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用,柱内径可从微径柱(1.0mm 内径)扩展到制备尺寸(50mm 内径)。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相(反相酰胺)。

Ascentis Phenyl 苯基柱在反相模式(包括 100% 水性条件)下具有出色的分离能力。也可用于 HILIC/ANP(水性正相)模式,并在梯度应用时紫外/质谱流失低。

特點和優勢

  • 100% 水性兼容
  • ANP/HILIC 和反相
  • 梯度条件下的低紫外/质谱流失
  • 交替选择性

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法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar

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