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用途
sufficient for 1000 extractions
sufficient for 5000 amplifications
顏色
colorless
運輸包裝
wet ice
儲存溫度
−20°C
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一般說明
Extract-N-Amp™血液PCR试剂盒提供了从全血快速提取DNA以及通过直接PCR扩增靶标所需的所有试剂。这种新型提取系统无需进行任何类型的纯化、有机提取、离心、加热、过滤或酒精沉淀。该试剂盒还包括专门配制的PCR ReadyMix™，可直接从提取物中扩增。这种配方使用基于抗体的热启动，用于特异性扩增。
應用
Extract-N-Amp™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used in polymerase chain reaction (PCR).
特點和優勢
- 高效8分钟预制品允许更大的速度和吞吐量
- 不需要任何类型的净化、有机提取、离心或酒精沉淀
- 简单的3步程序，无需特殊设备
- 包括Hot start抗体，用于基因组DNA的高特异性PCR扩增
- 兼容多种格式（单管或96孔板）
- 可与全血或血卡一起使用
- 在4℃稳定提取至少6个月
原則
基因组DNA从10 μl全血中提取，只需加入提取液并在室温下孵育5分钟。PCR前，将中和液添加至提取液以中和抑制物质。然后将一部分DNA提取物添加至专门配制的PCR Mix的PCR ReadyMix中。
其他說明
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
法律資訊
购买本产品包括豁免根据产品说明书中所规定的专利提起的诉讼，仅可将所购买的量用于购买者自己的内部研究。′其他专利权（例如5′核酸酶工艺的专利权）不得明示、暗示或禁止反言。 有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
根据美国专利号5,338,671和5,587,287以及其他国家的相应专利批准用于体外研究的抗体。
根据美国专利号5,338,671和5,587,287以及其他国家的相应专利批准用于体外研究的抗体。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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