V9139

Anti-Visfatin (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 别名: Anti-NAmPRTase, Anti-NMPT, Anti-Nicotinamide-phosphoribosyltransferase, Anti-PBEF1, Anti-Pre-B-cell colony enhancing factor 1
• UNSPSC代码: 12352203

属性

• 生物来源: rabbit
• 偶联物: unconjugated
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 表单: buffered aqueous solution
• 分子量: antigen ~50 kDa
• 种属反应性: rat, human, mouse (predicted)
• 浓度: ~1.5 mg/mL
• 技术: western blot: 1-2 μg/mL using A549 cell lysate; western blot: 2-4 μg/mL using rat lung extract (S1 fraction)
• UniProt登记号: P43490
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... NAMPT(10135) ; mouse ... Nampt(59027) ; rat ... Nampt(297508)

说明

一般描述

The gene for visfatin (also known as pre-B colony enhancing factor 1, PBEF1, nicotinamide phosphoribosyl transferase, NAMPT), is mapped to human chromosome 7q22.3. The encoded protein is an adipokine produced from adipocytes. It is a highly conserved protein and is also secreted by lymphocytes. Visfatin is expressed in large amounts in the bone marrow, liver tissue, and muscle and is also present in heart, placenta, lung, and kidney tissues.

特异性

Anti-Visfatin (C- terminal) recognizes human and rat visfatin.

应用

Anti-Visfatin (C-terminal) antibody produced in rabbit has been used in:

• western blotting(10)
• immunohistochemistry
• immunofluorescence

生化/生理作用

Visfatin is an enzyme that catalyzes the biosynthesis of nicotinamide adeninine dinucleotide (NAD) from nicotinamide. A variety of signaling pathways are regulated by visfatin including phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT), signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated protein kinase (ERK1/2). It is also reported to regulate the activity of sirtuin 1 (SIRT1) and protect the mammalian cells from death. Since visfatin catalyses a major rate-limiting step in the production of NAD, it is crucial in metabolism, carcinogenesis and immune responses. Visfatin also can act as cytokine and regulates the expression of vascular endothelial growth factor (VEGF) in the proliferation of endothelial cells and formation of capillary tubes. Deregulation of visfatin has been implicated in diabetes mellitus, obesity, rheumatoid arthritis and chronic hepatitis

Visfatin is involved in the regulation of energy homeostasis. Visfatin has been shown to activate its target cells by binding to the insulin receptor at a site distinct from insulin, and its expression level in plasma increases during the development of obesity. Visfatin can induce the cellular expression of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-7,(11) and IL-6.

目标描述

Visfatin (C-terminal) (also known as pre-B colony enhancing factor1, PBEF1, nicotinamide phosphoribosyltransferase, NAMPT), is a highly conserved protein involved in the regulation of cellular metabolism and energy homeostasis.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable
• 个人防护装备: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)