推荐产品
生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen 46 kDa (additional band of ~105 kDa may be observed in some preparations)
物種活性
mouse, rat, human
濃度
~1.5 mg/mL
技術
western blot: 0.4-0.8 μg/mL using rat brain extract (S1 cytosolic fraction)
western blot: 1-2 μg/mL using cell lysates of mouse fibroblast NIH3T3 cells and human A431 cells
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... TSG101(7251)
mouse ... Tsg101(22088)
rat ... Tsg101(292925)
一般說明
Tumor susceptibility gene 101 encodes a multi-domain binding protein TSG101 which is 46 kDa. It is expressed in all tissues throughout development and is essential for both embryonic development and normal cell growth. It has an N-terminal UEV (N-terminal ubiquitin E2 variant) domain. Subcellular localization of TSG101 fluctuates in a cell-cycle dependent manner being localized in the nucleus and Golgi complex during interphase and in mitotic spindles and centrosome during mitosis.
免疫原
synthetic peptide corresponding to amino acids 371-390 located at the C-terminus of human TSG101, conjugated to KLH. This sequence is identical in rat and mouse TSG101.
應用
Anti-TSG101 (C-terminal) antibody has been used in immnunoblotting and western blotting.
生化／生理作用
TSG101 mediates a variety of cellular functions including transcriptional regulation, ubiquitination, endosomal trafficking, proliferation, and cell survival. It has a central role in protein sorting during endocytosis. Human TSG101 is also required for the budding of several enveloped RNA viruses including human immunodeficiency (HIV) and Ebola viruses. TSG101 is involved in the regulation of the p53/MDM2 (proto-oncogene) pathway by affecting E3 ubiquitin-protein ligase MDM2 (MDM2) stability. The N-terminal UEV domain binds to a P(T/S)AP sequence of HIV-1 p6 Gag and Ebola Vp40 proteins required for budding. The proline-rich domain was found to act as an activation domain in transcriptional regulation. The C-terminal coiled-coil (CC2) domain was predicted to interact with the cell growth regulating protein stathmin and is involved in potential co-repressor activity.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Tumor susceptibility gene 101 protein represses androgen receptor transactivation and interacts with p300
Sun Z, et al.
Cancer, 86(4), 689-696 (1999)
Tsg101 is essential for cell growth, proliferation, and cell survival of embryonic and adult tissues
Wagner K, et al.
Molecular and Cellular Biology, 23(1), 150-162 (2003)
Let-7 microRNA family is selectively secreted into the extracellular environment via exosomes in a metastatic gastric cancer cell line
Ohshima K, et al.
PLoS ONE, 5(10), e13247-e13247 (2010)
Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding
Garrus JE, et al.
Cell, 107(1), 55-65 (2001)
Joanna Kowal et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(8), E968-E977 (2016-02-10)
Extracellular vesicles (EVs) have become the focus of rising interest because of their numerous functions in physiology and pathology. Cells release heterogeneous vesicles of different sizes and intracellular origins, including small EVs formed inside endosomal compartments (i.e., exosomes) and EVs
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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