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一般說明
胞苷脱氨酶(CDA)是一种清除外源和内源性胞苷和2′-脱氧胞苷的酶,可用于UMP合成。该蛋白是负责维持细胞嘧啶池的几种脱氨酶之一。CDA还可催化化学疗法胞嘧啶核苷类似物(如阿拉伯呋喃糖基胞苷(Ara-C)和5-氮胞苷)的脱氨基反应,从而导致其细胞毒性和抗肿瘤功能丧失。
生化/生理作用
胞嘧啶脱氨酶(CDA)主要在粒细胞中表达,可以形成同源四聚体。在N端与His标签融合的重组人CDA蛋白,其在大肠杆菌中表达并通过常规色谱进行纯化。
外觀
0.5 mg/mL,溶于含有100 mM NaCl、1 mM DTT、2 mM EDTA和40%甘油的20 mM Tris-HCl缓冲液(pH 8.0)中。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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