推荐产品
生物来源
human
重组
expressed in HEK 293 cells
偶联物
(Glycosaminoglycan)
标签
6-His tagged (C-terminus)
方案
≥92% (SDS-PAGE)
表单
lyophilized powder
分子量
calculated mol wt 52.1 kDa
observed mol wt 64-66 kDa (DTT-reduced. Protein migrates due to different glycosylation. Leu 36 is the predicted N-terminus.)
包装
pkg of 10 μg
pkg of 50 μg
杂质
<1 EU/μg endotoxin (LAL test)
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
human ... SPAM1(6677)
一般描述
Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1→4)-linkages between N – acetyl – β – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
外形
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.0-7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
重悬
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
其他客户在看
Andrée-Anne Saindon et al.
Reproduction (Cambridge, England) (2018-10-18)
Sperm adhesion molecule 1 (SPAM1) is a sperm protein possessing a hyaluronidase domain in its N-terminus and a zona pellucida-binding domain in its C-terminus. Our previous studies showed that bovine spermatozoa potentially have 2 SPAM1 isoforms that present different C-terminal
Dong Leng et al.
BioMed research international, 2020, 3659451-3659451 (2020-04-08)
Idiopathic pulmonary fibrosis (IPF), the most common interstitial lung disease, arises from transforming growth factor beta 1- (TGFβ1-) induced aberrant fibroproliferation in response to epithelial injury. The TGFβ1-) induced aberrant fibroproliferation in response to epithelial injury. The TGF. We first
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