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一般說明
Sigma mirPremier microRNA分离试剂盒可以快速、有效地从不同的生物学来源样本，包括哺乳动物细胞培养物、动物组织、植物组织、微生物培养物中纯化和富集miRNA和其他小分子RNA，且无需进行任何有害的有机萃取。microRNA (miRNA) 属于小分子RNA，长度大约为21个核苷酸(nt)，通过各种方式调节着基因表达，包括翻译抑制、mRNA切割和脱腺苷化。此外，如果您关注信使RNA和其它大分子RNA，这种试剂盒还可用于分离总RNA。
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應用
mirPremier® microRNA分离试剂盒已被用于：
- 以来源于可食用植物葡萄的类外泌体纳米颗粒（EPDEN）提取含miRNA 的总RNA 。
- 从S.sirkka 、S. napiecek和S. arctica中分离小分子RNA。
- 从冷冻大鼠肝脏中分离miRNA用于miRNA 分析。
特點和優勢
- 旨在提高各种生物学来源的miRNA和其它小RNA分子的分离效率
- 在30分钟内为下游应用实现快速而高效的miRNA提取和浓缩
- 可以提取不含大RNA的高纯度miRNA
- 不涉及危险的有机提取
法律資訊
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險分類
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Mu J
Molecular Nutrition And Food Research, 58(7), 1561-1573 (2014)
Dongxiao Su et al.
Food & function, 8(2), 808-815 (2017-01-26)
Dietary phenolics exhibit hypolipidemic activity by changing lipid metabolism-related microRNA (miRNA) expression. Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase (quercetin 3-rut-7-rha), rutin and (-)-epicatechin are the main phenolics in lychee (Litchi chinensis Sonn.) pulp. A previous study reported that quercetin 3-rut-7-rha and rutin had hypolipidemic
Jon Bråte et al.
Current biology : CB, 28(20), 3288-3295 (2018-10-16)
The emergence of multicellular animals was associated with an increase in phenotypic complexity and with the acquisition of spatial cell differentiation and embryonic development. Paradoxically, this phenotypic transition was not paralleled by major changes in the underlying developmental toolkit and
Abdulla Abdulla Sabana et al.
Planta, 251(4), 79-79 (2020-03-14)
Genome-wide analysis of small RNAs identifies somatic embryogenesis- specific miRNAs and their targets and provides novel insights into the mechanisms governing somatic embryogenesis in coconut, a highly in vitro recalcitrant species. Coconut, a major plantation crop of the tropics is
Unicellular origin of the animal microRNA machinery
Br?te J, et al.
Current Biology, 28(20), 3288- 3295 (2018)
商品
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。
相关内容
Sigma-Aldrich® Advanced Genomics是CRISPR、Cas9、合成向导RNA(sgRNA)和锌指核酸酶(ZFN)等基因编辑和沉默技术的领先提供者。
Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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