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一般說明
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases. This product is free of detectable microbial DNA and is suitable for microbiome research
應用
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
生化／生理作用
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. Optimum pH is 8.5-9.
特點和優勢
Contains two bacteriolytic proteases for efficient lysis of Gram-positive bacteria and Gram-negative organisms
Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods
Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA
Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies
Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods
Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA
Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies
單位定義
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
商品
裂解酶提供了一种非机械式细胞裂解和原生质体准备方法。这看上去是个非常简单的过程：只需加入酶，将试管放入水浴然即可。但这过程中到底发生了什么？
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
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