推荐产品
生物来源
mouse
质量水平
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
PC 10, monoclonal
表单
buffered aqueous solution
分子量
antigen ~36 kDa
种属反应性
monkey, rat, insect, mouse, yeast, human
浓度
~1.0 mg/mL
技术
ELISA: suitable
flow cytometry: suitable
immunoblotting: 0.5-1 μg/mL using human foreskin fibroblast HS-68 cell line extract
immunofluorescence: 1.25-2.5 μg/mL using HeLa cells
immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human Tonsil sections.
immunoprecipitation (IP): suitable
同位素/亚型
IgG2a
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... PCNA(5111)
一般描述
Anti-Proliferating Cell Nuclear Antigen antibody (mouse IgG2a isotype) is derived from the PC 10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. PCNA is an auxiliary protein of DNA polymerase δ and belongs to the conserved sliding clamp family of proteins.
PCNA (proliferating cell nuclear antigen) gene is mapped in human chromosome 20p12.3. PCNA is a homotrimeric protein, which is expressed in the nucleus. The PCNA ring has a large central cavity that allows double-stranded DNA to slide freely along it.
特异性
Monoclonal Anti-Proliferating Cell Nuclear Antigen (PCNA) specifically recognizes Proliferating Cell Nuclear Antigen from human, monkey, rat, mouse, insect and yeast origin.
免疫原
PCNA-Protein A fusion protein.
应用
Anti-Proliferating Cell Nuclear Antigen antibody, Mouse monoclonal may be used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- flow cytometry
- immunohistochemistry
- immunoprecipitation
- immunofluorescence
生化/生理作用
PCNA (proliferating cell nuclear antigen) maintains the polymerase and DNA association by forming a sliding clamp and ensures efficient DNA replication. PCNA is responsible for recruiting important regulatory factors for DNA damage tolerance mechanism. In response to UV irradiation-induced DNA damage, monoubiquitination of PCNA mediates the polymerase switching model by recruiting DNA polymerase η to PCNA. The protein is involved in base excision repair and maintains genome stability. PCNA is associated with proteolysis coupled to DNA synthesis and repair for proper cell cycle progression.
PCNA protein is essential for chromosomal DNA replication during S-phase and is required for several DNA processes like DNA repair, sister chromatid cohesion and cell cycle control. This protein is also involved in the epigenetic modification and chromatin assembly and remodeling. PCNA acts as a prognostic and diagnostic marker in several diseases such as breast cancer, chronic lymphoid leukemia, non-Hodgkin′s lymphoma, malignant and nonmalignant skin diseases and systemic lupus erythematosus.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Replication Protein A Prohibits Diffusion of the PCNA Sliding Clamp along Single-Stranded DNA.
Hedglin M
Biochemistry (2017)
J E Celis et al.
FEBS letters, 220(1), 1-7 (1987-08-10)
Cyclin, also known as PCNA or the auxiliary protein of mammalian DNA polymerase delta, is a stable cell cycle regulated (synthesized mainly in S-phase) nuclear protein of apparent Mr 36,000 whose rate of synthesis correlates directly with the proliferative state
Karin M Gilljam et al.
The Journal of cell biology, 186(5), 645-654 (2009-09-09)
Numerous proteins, many essential for the DNA replication machinery, interact with proliferating cell nuclear antigen (PCNA) through the PCNA-interacting peptide (PIP) sequence called the PIP box. We have previously shown that the oxidative demethylase human AlkB homologue 2 (hABH2) colocalizes
Proliferating cell nuclear antigen prevents trinucleotide repeat expansions by promoting repeat deletion and hairpin removal.
Beaver JM
DNA Repair (2016)
Shao-Chun Wang
Trends in pharmacological sciences, 35(4), 178-186 (2014-03-25)
Proliferating cell nuclear antigen (PCNA) is known as a molecular marker for proliferation given its role in replication. Three identical molecules of PCNA form a molecular sliding clamp around the DNA double helix. This provides an essential platform on which
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