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生物来源
rat
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
BRM 2D3, monoclonal
表单
buffered aqueous solution
分子量
antigen ~80 kDa
种属反应性
rat, mouse, monkey, human, bovine, canine
包装
antibody small pack of 25 μL
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using HeLa cells extract
同位素/亚型
IgG2a
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... BRD7(29117)
一般描述
Monoclonal Anti-BRD7 (rat IgG2a isotype) is derived from the hybridoma BRM 2D3 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a fusion protein expressing a fragment of human BRD7. Bromodomain containing (BRD7) is a novel bromodomain gene.
应用
Monoclonal Anti-BRD7 antibody produced in rat has been used in several immunochemical techniques including
- immunoblotting
- immunoprecipitation
- immunocytochemistry
生化/生理作用
Bromodomain containing (BRD7) inhibits cell growth and cell cycle progression by transcriptional regulation of some cell cycle-related genes as well as some important molecules involved in ras/mitogen-activated protein kinase(MEK)/extracellular-signal-regulated kinase (ERK) and retinoblastoma tumor suppressor protein (RB)/ E2 factor (E2F) pathways. Its transcriptional down regulation has been shown to be critical to the pathogenesis of nasopharyngeal carcinoma (NPC). Specifically, DNA methylation decreases the expression of BRD7 in NPC cells. Furthermore, DNA methylation of BRD7 promoter is increased in the tumor and in matched blood samples from NPC patients than in blood samples from healthy individuals. Therefore, it has been suggested that DNA methylation of BRD7 promoter might act as a potential diagnostic marker in NPC.
外形
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
Promoter methylation inhibits BRD7 expression in human nasopharyngeal carcinoma cells
Liu H, et al.
BMC Cancer, 8(1), 253-253 (2008)
Nasopharyngeal carcinoma-review of the molecular mechanisms of tumorigenesis
Chou J, et al.
Head & Neck, 30(7), 946-963 (2008)
The transcriptional regulation role of BRD7 by binding to acetylated histone through bromodomain
Cong P, et al.
Journal of Cellular Biochemistry, 97(4), 882-892 (2006)
Shao-An Wang et al.
Scientific reports, 10(1), 20870-20870 (2020-12-02)
Bromodomain (BRD)-containing proteins are important for chromatin remodeling to regulate gene expression. In this study, we found that the deubiquitinase USP24 interacted with BRD through its C-terminus increased the levels of most BRD-containing proteins through increasing their protein stability by
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