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生物源
chicken
共軛
peroxidase conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
lyophilized powder
物種活性
rabbit
技術
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
一般說明
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical (called Fab fragments) and contain the antigen-binding activity. The third fragment (called Fc fragment) does not possess antigen-binding activity, but binds to cells and effector molecules. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants.
特異性
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Chicken Serum, Rabbit IgG and Rabbit Serum.
免疫原
Rabbit IgG whole molecule
應用
Anti-Rabbit IgG (H+L)-Peroxidase antibody produced in chicken has been used in Western blotting.
物理性質
Antibody format: IgG
外觀
Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
重構
Reconstitute with 1.0 mL deionized water (or equivalent).
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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