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用途
sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)
技術
protein staining: suitable
儲存溫度
2-8°C
應用
R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.
其他說明
Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.
仅试剂盒组分
产品编号
说明
- Solution A 500 mL
- Solution B 500 mL
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
儲存類別代碼
8B - Non-combustible corrosive hazardous materials
其他客户在看
Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)
商品
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。
实验方案
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
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