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product name
RPMI-1640 培养基, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
無菌
sterile-filtered
形狀
liquid
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
pH值
>7.2
成分
sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
應用
RPMI-1640 Medium has been used
- to obtain spleen and lymph nodes from mice
- for the resuspension of Staphylococcus aureus strains in the phagocytosis assay
- in the isolation of hepatitis B surface antigen-specific B-cell clones
重構
Supplement with 0.3 g/L L-glutamine.
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产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Kamila R Santos et al.
Vaccines, 9(8) (2021-08-29)
Staphylococcus aureus mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit α (SAS), succinyl-diaminopimelate (SDD), and
Blood polymorphonuclear leukocyte responses against Staphylococcus aureus in primiparous and pluriparous Lacaune and Santa Ines ewes
Marques M, et al.
Small Ruminant Research, 201, 106412-106412 (2021)
Vitória M Silva et al.
BMC veterinary research, 17(1), 282-282 (2021-08-27)
The present study explored the viability of bovine milk macrophages, their intracellular production of reactive oxygen and nitrogen species (RONS), and their phagocytosis of Staphylococcus aureus, as well as the profile of lymphocytes, from healthy udder quarters and udder quarters
Ewerton de Souza Lima et al.
Frontiers in veterinary science, 8, 650021-650021 (2021-07-06)
The implications of bovine leukemia virus (BLV) on innate and adaptive immune responses have been widely investigated; however, the effects of BLV on mammary gland immunity require further investigation. The present study investigated the viability, phagocytic capacity, and intracellular production
Antonella Cerino et al.
PloS one, 10(4), e0125704-e0125704 (2015-04-30)
We describe the production and characterization of human monoclonal antibodies (mAb) specific for the major hepatitis B virus (HBV) S protein. The mAbs, two IgG1κ and one IgG1λ, were secreted by B-cell clones obtained from peripheral blood mononuclear cells (PBMC)
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