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应用
该试剂盒用于标记吞噬细胞。可以选择性标记具有吞噬能力的细胞,例如单核细胞、巨噬细胞或中性粒细胞。
原理
吞噬细胞的具体标记方法是形成染料聚集体或颗粒物。这样可以显著抑制淋巴细胞等非吞噬细胞对染料的吸收,同时促进吞噬细胞对染料的吸收。因为染料聚集于这些细胞的吞噬小体内,标记后的细胞呈片状或点状色斑。这些染料不易被细胞代谢清除,可在体内保持至少21天。
这种方法标记吞噬细胞可同时在体外或体内进行。通过腹膜内或静脉内注射PKH26标记溶液来完成可完成体内标记,而从体内分离出来的靶标细胞则可通过体外 标记方法进行染色。
这种方法标记吞噬细胞可同时在体外或体内进行。通过腹膜内或静脉内注射PKH26标记溶液来完成可完成体内标记,而从体内分离出来的靶标细胞则可通过体外 标记方法进行染色。
联系
有关PKH Fluorescent Cell Linker Dyes的其他技术信息,包括完整的参考书目,请访问这里。
法律信息
CellVue is a registered trademark of Phanos Technologies
仅试剂盒组分
产品编号
说明
- Diluent B 6 x 10
- PKH26 cell linker in ethanol .5 mL
警示用语:
Danger
危险声明
危险分类
Eye Irrit. 2 - Flam. Liq. 2
储存分类代码
3 - Flammable liquids
闪点(°F)
57.2 °F - closed cup
闪点(°C)
14.0 °C - closed cup
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