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品質等級
儲存溫度
2-8°C
一般說明
PeroxiDetect 试剂盒可用于测定水溶性过氧化物和脂质过氧化物，专为检测生物系统中的过氧化物而研发，后者是特定组织中自由基水平的重要决定因素。
應用
PeroxiDetect™试剂盒适用于：
- 突触体制备中氢过氧化物的定量分析
- 测定黑素瘤细胞脂质过氧化反应
- 大脑皮层中过氧化物的定量
- 肠腔样品
在酸性条件下，过氧化物可将Fe2+ 离子转化为3+ 离子。Fe3+ 离子可与二甲酚橙形成可在560 nm 下观察的有色复合物。
用于测定水溶性过氧化物和脂质过氧化物。过氧化物是羟基或过氧化活性自由基的来源。这些自由基可与细胞中的DNA、蛋白质或脂质成分反应，引起细胞损伤，进而导致细胞死亡。对生物系统中过氧化物的检测是确定特定组织中自由基水平的重要因素。研究表明，脂质过氧化物是引起多种病理生理学细胞和组织异常的原因。例如，已发现红细胞中脂质过氧化产物的增加与孕妇糖尿病的发生相关。血液中酒精含量过高的患者会出现胆固醇氢过氧化物堆积。对组织内过氧化氢的检测，已被用于研究自由基损伤的几个方面，如UV引起的皮肤老化以及H2O2 对黑色素瘤细胞中酪氨酸酶水平增加的诱导作用。H2O2 已被证明是限制生长培养条件下人主动脉平滑肌细胞的有效分裂原。PeroxiDetect 试剂盒能够在水溶液中以每反应体积1-7 nM 的范围内检测H2O2，或在有机溶剂中以每反应体积1-16 nM 范围内检测脂质过氧化物。在酸性条件下，过氧化物可将2+ 离子转化为Fe3+ 离子，该试剂盒正是基于这一原理而研制的。Fe3+ 离子可与二甲酚橙形成可在560 nm 下观察的有色复合物。
成分
试剂盒包含了可用于进行100次水溶性过氧化物检测和100次有机过氧化物检测的试剂。
法律資訊
PeroxiDetect is a trademark of Sigma-Aldrich Co. LLC
仅试剂盒组分
产品编号
说明
- tert-Butyl hydroperoxide 1 mL
- Hydrogen peroxide 1 mL
訊號詞
Danger
危險分類
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Org. Perox. C - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
5.2 - Organic peroxides and self-reacting hazardous materials
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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E Karg et al.
The Journal of investigative dermatology, 100(2 Suppl), 209S-213S (1993-02-01)
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