共軛
peroxidase conjugate
品質等級
100
200
形狀
lyophilized powder
技術
direct ELISA: 1:80,000 using human IgG
儲存溫度
−20°C
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一般說明
蛋白A是金黄色葡萄球菌的细胞壁成分,分子量为56kDa。它具有五个结合结构域,可与Fc或Fab片段的免疫球蛋白重链发生相互作用。蛋白A可激活补体系统、干扰素产生、超敏反应和细胞介导的细胞毒性作用。辣根过氧化物酶是一种含血红素的酶。 在过氧化氢存在下,该酶催化鲁米诺的氧化。
特異性
仅与大多数哺乳动物的IgG结合,除了大鼠、山羊、绵羊。
應用
来自金黄色葡萄球菌/辣根的蛋白A-过氧化物酶已用于酶联免疫吸附测定(ELISA)。
包裝
包装规格基于蛋白含量。
外觀
冻干粉末,含有柠檬酸钠缓冲盐。通过A205测定蛋白质。
準備報告
通过改良O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978)中所报道的有利于低分子量偶联物的实验方法,使用VI型过氧化物酶标记细胞外蛋白A。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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实验方案
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
该实验程序用于使用连苯三酚作为底物测定过氧化物酶的酶活性。
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