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Merck

P5459

Sigma-Aldrich

蛋白酶 来源于地衣芽孢杆菌

glycerol solution (50%)

别名:

Subtilisin A, Subtilisin Carslberg, 枯草杆菌蛋白酶A, 蛋白酶 来源于地衣芽孢杆菌

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物源

Bacillus licheniformis

形狀

glycerol solution (50%)

比活性

≥7 units/mg protein (biuret)

濃度

≥50 mg/mL

溶解度

0.85% sodium chloride: soluble, clear (Diluted)
H2O: miscible

儲存溫度

2-8°C

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一般說明

该酶在50℃下、pH 8-10的范围内具有最佳的明胶水解效果。商业上,它在50℃下、pH 6-10之间是稳定的,被用于用于洗衣洗涤剂配方,也用于从照相乳剂中回收银。

應用

使用来自地衣芽孢杆菌的蛋白酶来消化蛾触角叶中的神经外膜;绿棉铃虫
蛋白酶是一种通过水解肽键来分解蛋白的酶。蛋白酶用于降解蛋白质,研究蛋白酶抑制剂和研究热失活动力学。 蛋白酶用于孵育中的核酸分离步骤。来自地衣芽孢杆菌的蛋白酶(商品货号P5459)用于从骨胳肌中分离线粒体时水解肌肉。

生化/生理作用

蛋白酶通过肽键的水解使蛋白质分解代谢。蛋白酶可被丝氨酸活性位点抑制剂所灭活,如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。

包裝

包装规格基于蛋白含量。

單位定義

若无其他说明,则一个酶活性单位是指在pH7.5、37℃条件下每分钟水解酪蛋白并产生相当于1.0 μM (181 μg) 酪氨酸的颜色(使用Folin-Ciocalteu试剂显色)所需的酶量。

象形圖

Health hazardCorrosion

訊號詞

Danger

危險聲明

危險分類

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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