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一般說明
该酶在50℃下、pH 8-10的范围内具有最佳的明胶水解效果。商业上,它在50℃下、pH 6-10之间是稳定的,被用于用于洗衣洗涤剂配方,也用于从照相乳剂中回收银。
應用
使用来自地衣芽孢杆菌的蛋白酶来消化蛾触角叶中的神经外膜;绿棉铃虫。
蛋白酶是一种通过水解肽键来分解蛋白的酶。蛋白酶用于降解蛋白质,研究蛋白酶抑制剂和研究热失活动力学。 蛋白酶用于孵育中的核酸分离步骤。来自地衣芽孢杆菌的蛋白酶(商品货号P5459)用于从骨胳肌中分离线粒体时水解肌肉。
生化/生理作用
蛋白酶通过肽键的水解使蛋白质分解代谢。蛋白酶可被丝氨酸活性位点抑制剂所灭活,如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。
包裝
包装规格基于蛋白含量。
單位定義
若无其他说明,则一个酶活性单位是指在pH7.5、37℃条件下每分钟水解酪蛋白并产生相当于1.0 μM (181 μg) 酪氨酸的颜色(使用Folin-Ciocalteu试剂显色)所需的酶量。
訊號詞
Danger
危險聲明
危險分類
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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