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生物源
mouse
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
PRK8, monoclonal
形狀
buffered aqueous solution
物種活性
mouse, rat, hamster, human
濃度
~2 mg/mL
技術
microarray: suitable
western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract
同型
IgG2b
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... PARK2(5071)
mouse ... Park2(50873)
rat ... Park2(56816)
一般說明
Parkin 是一种泛素蛋白连接酶,在许多组织中表达,例如大脑、睾丸、骨骼肌和心脏。它是一种 RING 型 E3 泛素蛋白连接酶,可促进错误折叠蛋白的泛素化,并防止这些蛋白引起的神经毒性作用。抗 Parkin 单克隆抗体可用于微阵列和免疫沉淀测定。它还可用于蛋白印迹分析。小鼠抗 Parkin 抗体与大鼠、仓鼠、小鼠和人类的 Parkin(约 50kD)特异性反应。
抗 Parkin单克隆抗体(小鼠 IgG2b 同种型)源自杂交瘤 PRK8,该杂交瘤由小鼠骨髓瘤细胞(SP2 细胞)和重组人 Parkin 免疫的 BALB/c 小鼠的脾细胞融合产生。Parkin 是一种泛素蛋白连接酶,在许多组织中表达,例如大脑、睾丸、骨骼肌和心脏。Parkin 基因编码一个由 465 个氨基酸残基组成的蛋白质,其氨基末端与泛素具有中等相似性,而羧基末端则有一个无名指间环 (IBR)-无名指基序。该基因有12个外显子。
免疫原
重组人Parkin。
應用
Parkin单克隆抗体可用于:
- 酶联免疫吸附测定(ELISA)
- 免疫印迹
- 免疫沉淀[63}
- 免疫细胞化学
- 在pull-down测定中标记Parkin
外觀
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Heart failure, mostly associated with cardiac hypertrophy, is a major cause of illness and death. Oxidative stress causes accumulation of reactive oxygen species (ROS), leading to mitochondrial dysfunction, suggesting that mitochondria-targeted therapies could be effective in this context. The purpose
Genomic organization and expression of parkin in Drosophila melanogaster
Experimental & Molecular Medicine, 35(5), 393-393 (2003)
Parkin regulates kainate receptors by interacting with the GluK2 subunit
Nature Communications, 5(46) (2014)
The Journal of biological chemistry, 290(42), 25199-25211 (2015-08-12)
Damaged mitochondria are eliminated through autophagy machinery. A cytosolic E3 ubiquitin ligase Parkin, a gene product mutated in familial Parkinsonism, is essential for this pathway. Recent progress has revealed that phosphorylation of both Parkin and ubiquitin at Ser(65) by PINK1
Molecular and cellular biology, 35(1), 211-223 (2014-10-29)
Mitochondria are dynamic organelles, and their fusion and fission regulate cellular signaling, development, and mitochondrial homeostasis, including mitochondrial DNA (mtDNA) distribution. Cardiac myocytes have a specialized cytoplasmic structure where large mitochondria are aligned into tightly packed myofibril bundles; however, recent
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