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Merck

P1034

Sigma-Aldrich

异硫氰酸苯酯

Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid

别名:

PITC

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About This Item

线性分子式:
C6H5NCS
CAS号:
分子量:
135.19
Beilstein:
471392
EC號碼:
MDL號碼:
分類程式碼代碼:
12352118
PubChem物質ID:
NACRES:
NA.31

等級

Sigma Grade

品質等級

化驗

≥99% (GC)

形狀

liquid

濃度

8.36 M

折射率

n20/D 1.6515 (lit.)

bp

218 °C (lit.)

mp

−21 °C (lit.)

密度

1.132 g/mL at 20 °C (lit.)

適合性

suitable for solid phase protein sequencing analysis

儲存溫度

2-8°C

SMILES 字串

S=C=Nc1ccccc1

InChI

1S/C7H5NS/c9-6-8-7-4-2-1-3-5-7/h1-5H

InChI 密鑰

QKFJKGMPGYROCL-UHFFFAOYSA-N

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一般說明

异硫氰酸苯酯属于带异硫氰酸基团的芳香族化合物类。它通常用作柱前衍生剂,用于形成苯硫代氨基甲酰基氨基酸,以便通过色谱技术进行测定。

應用

异硫氰酸苯酯可用于通过自动埃德曼反应和之后的高效液相色谱分析(HPLC)技术,在亚皮摩尔和亚纳摩尔水平进行多肽和蛋白质测序。
用于伯胺和仲胺的衍生化试剂。用于通过 Edman 降解法进行多肽测序以及通过 HPLC 进行氨基酸分析。

包裝

氩气下用火焰封装的 1mL 棕色安瓿瓶包装。
氩气下装瓶的 10mL 螺纹盖棕色样品瓶包装。

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

190.4 °F - closed cup

閃點(°C)

88 °C - closed cup

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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R L Heinrikson et al.
Analytical biochemistry, 136(1), 65-74 (1984-01-01)
Methods for the quantitative derivatization of amino acids with phenylisothiocyanate and for the separation and quantitation of the resulting phenylthiocarbamyl derivatives by reverse-phase high-performance liquid chromatography are described. Phenylthiocarbamylation of amino acids proceeds smoothly in 5 to 10 min at
Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis
Tempst P and Riviere L
Analytical Biochemistry, 183(2), 290-300 (1989)
Additives for High Performance Applications: Chemistry and Applications
Additives for High Performance Applications: Chemistry and Applications, 235(1), 48-62 (2016)
The Protein Protocols Handbook, 235(1), 48-62 (1996)
H S Lu et al.
Archives of biochemistry and biophysics, 235(1), 48-62 (1984-11-15)
Automated solid-phase sequencing using 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate (DABITC) double coupling or regular phenylisothiocyanate (PITC) degradation procedures have been investigated. Employing sensitive high-performance liquid chromatography for the identification of amino acid thiohydantoin derivatives (PTH and DABTH), both methods were capable of sequencing

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