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用途
sufficient for 25 purifications
品質等級
環保替代產品特色
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技術
DNA purification: suitable
環保替代類別
, Aligned
儲存溫度
15-25°C
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一般說明
GenElute HP质粒中量制备试剂盒可以超快速、高效的从重组大肠杆菌培养物大批量制备质粒。该试剂盒结合了硅胶膜技术和离心或真空形式的便利性。该试剂盒还附带专用的滤器卡夹,从而替代了碱裂解之后的离心分离步骤。裂解之后,DNA结合到硅胶膜上,并通过一次简单的洗涤步骤去除了污染物。随后在水或TE缓冲液中,通过离心处理洗脱DNA。
纯化所得的质粒DNA适于包括限制性酶切和测序在内的一系列分子生物学应用。
纯化所得的质粒DNA适于包括限制性酶切和测序在内的一系列分子生物学应用。
我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。相比标准DNA提取方法使用苯酚和氯仿,该产品是一种更安全的化学产品。
應用
GenElute™ HP 质粒Midiprep试剂盒已用于分离和纯化质粒。
特點和優勢
- 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
- 可纯化出多达350 μg高拷贝数质粒DNA
- 真空或离心形式可灵活选择
- 无需进行苯酚/氯仿萃取或乙醇沉淀步骤
- 所含的塑料成分更少,减少了废物量
其他說明
更多信息,请见www.sigma-aldrich.com/genelutehp。
法律資訊
GenElute is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
標靶器官
Central nervous system
儲存類別代碼
3 - Flammable liquids
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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相关内容
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
从细胞、组织、血液、病毒等样品类型中提取和纯化基因组DNA、质粒DNA和总RNA的常用技术及其下游应用的概述。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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