推荐产品
生物源
bovine heart
形狀
ammonium sulfate suspension
比活性
2000-4000 units/mg protein (modified Warburg-Christian)
分子量
35 kDa
雜質
≤0.01% Glutamic-Oxalacetic Transaminase
≤0.01% Glutamic-Pyruvic Transaminase
儲存溫度
2-8°C
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一般說明
牛心脏的苹果酸脱氢酶在 NAD 结合活性位点包含一个组氨酸残基，该残基对活性至关重要。当该组氨酸突变时，观察到活性丧失。
苹果酸脱氢酶是一种细胞质同工酶，是三羧酸循环中的重要催化剂。
應用
苹果酸脱氢酶已用于评估流动注射系统的研究，用于在线监测生物过程中的富马酸。 它也曾被用于一项研究，调查一种寄生在德州花生上的根结线虫。
單位定義
在pH7.5,25℃条件下，一个单位每分钟可将1.0 μmol的草酰乙酸和β-NADH转化为 L苹果酸和β-NAD。
外觀
3 M (NH4)2SO4 - 0.01 M KH2PO4 溶液 (pH 7.3) 中的混悬液
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
实验方案
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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