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生物源
mouse
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
ERK-PY193, monoclonal
形狀
buffered aqueous solution
分子量
antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa
物種活性
rat, human
濃度
~2 mg/mL
技術
capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 1-5 μg/mL using cell extract of rat fibroblasts cell line, Rat1, activated with sorbitol
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
phosphorylation (pTyr)
基因資訊
human ... MAPK1(5594) , MAPK3(5595)
rat ... Mapk1(116590) , Mapk3(50689)
一般說明
Monoclonal Anti-MAP Kinase, Mono-phosphorylated Tyrosine (pY-ERK) (mouse IgG1 isotype) is derived from the ERK-PY193 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide sequence corresponding to the phosphorylated form of ERK-activation loop, conjugated to KLH. The mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in many signaling pathways. This family includes the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK (c-Jun N-terminal protein kinase, also termed stress-activated protein kinase, SAPK1), and p38 MAPK (also termed SAPK2) subfamilies.
特異性
The antibody reacts with the monophosphorylated tyrosine form of MAP kinases (ERK1 and ERK2). It does not recognize the non-phosphorylated, diphosphorylated, and the monophosphorylated threonine forms of ERK/MAP kinases, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (e.g.,Tyr185 in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK.
免疫原
synthetic peptide HTGFLTEpYVAT, corresponding to the phosphorylated form of ERK-activation loop.
應用
Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine antibody has been used in:
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- dot-blot
- immunocytochemistry
- western blotting
- immunofluorescence
生化／生理作用
MAPK kinase (MAPKK) is the immediate upstream activator of the MAPK, MAPKK kinase (MAP3K), and MAP3K kinase (MAP4K) for the enzymes further upstream, respectively. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs.
外觀
Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
準備報告
Prepared from a culture supernatant of bioreactor grown hybridoma.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Mammalian MAP kinase signalling cascades
Chang L and Karin M
Nature, 410(6824), 37-37 (2001)
Monophosphothreonyl extracellular signal-regulated kinases 1 and 2 (ERK1/2) are formed endogenously in intact cardiac myocytes and are enzymically active.
Sugden PH, et al.
Cellular Signalling, 23(2), 468-477 (2011)
Paradoxical results in perturbation-based signaling network reconstruction
Prabakaran S, et al.
Biophysical Journal, 106(12), 2720-2728 (2014)
Sudhakaran Prabakaran et al.
Biophysical journal, 106(12), 2720-2728 (2014-06-19)
Mathematical models are extensively employed to understand physicochemical processes in biological systems. In the absence of detailed mechanistic knowledge, models are often based on network inference methods, which in turn rely upon perturbations to nodes by biochemical means. We have
G Pearson et al.
Endocrine reviews, 22(2), 153-183 (2001-04-11)
Mitogen-activated protein (MAP) kinases comprise a family of ubiquitous proline-directed, protein-serine/threonine kinases, which participate in signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. MAP kinases lie in protein kinase cascades.
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