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Merck

M0275

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

别名:

MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

product name

Eagle最低必需培养基, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

無菌

sterile-filtered

形狀

liquid

濃度

10 ×

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

運輸包裝

ambient

儲存溫度

2-8°C

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應用

最低必需培养基 Eagle 已用于配制甲基丙烯酸丙烯酰胺(水凝胶)。它也已用于培养牛膝关节的软骨,随后该牛膝关节软骨可用于 MRI(磁共振成像)研究和组织学分析。
通常用于在FBS、小牛或马血清存在下培养贴壁细胞,如成纤维细胞。待培养基稀释到1x和调节pH后,再添加L-谷氨酰胺和碳酸氢钠。

重構

向 1× 培养基中添加 0.292g/L L-谷氨酰胺、2.2g/L 碳酸氢钠。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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