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Merck
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文件

H9914

Millipore

HIS-Select® 镍磁性琼脂糖珠

别名:

nickel charged magnetic beaded agarose

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

共軛

magnetic beads

形狀

suspension

儲存期限

2 yr (Unopened product)

基質

6% beaded magnetic agarose

容量

≥15 mg/mL binding capacity

儲存溫度

2-8°C

相关类别

一般說明

HIS-选择性磁性琼脂糖珠由顺磁性的固定化金属离子亲和色谱（IMAC）树脂组成，包含专有的四配位体螯合物，与镍结合，通过不带电荷的亲水性连接子共价附于磁性珠状琼脂糖上。磁珠的磁性有助于操作，例如可反复清洗和回收与蛋白结合的磁珠。这使得实验重现性更高，更准确地定量组氨酸标记的目标蛋白。

應用

HIS-选择性镍磁性琼脂糖珠设计用于组氨酸标记蛋白的自动化和小规模亲和捕获（分子pull-down）纯化，同时表现出与其他蛋白非特异性结合低。该珠可在天然和变性条件下纯化His标签蛋白。

外觀

以30%乙醇溶液中的50%浆液悬浮液的形式提供。

法律資訊

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

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说明
价格

訊號詞

Danger

危險分類

Aquatic Chronic 3 - Carc. 1B - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2

標靶器官

Respiratory Tract

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

86.0 °F

閃點（°C）

30 °C

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Sonja Hänzelmann et al.
Clinical epigenetics, 7, 19-19 (2015-03-13)
Primary cells enter replicative senescence after a limited number of cell divisions. This process needs to be considered in cell culture experiments, and it is particularly important for regenerative medicine. Replicative senescence is associated with reproducible changes in DNA methylation
Mei Suen Kong et al.
Science signaling, 12(567) (2019-02-07)
T cell activation is initiated by signaling molecules downstream of the T cell receptor (TCR) that are organized by adaptor proteins. CIN85 (Cbl-interacting protein of 85 kDa) is one such adaptor protein. Here, we showed that CIN85 limited T cell
Joshua W Burgess et al.
American journal of respiratory cell and molecular biology, 41(6), 714-721 (2009-03-17)
Pneumocystis organisms are opportunistic fungal pathogens that cause significant pneumonia in immune-compromised hosts. Recent evidence has suggested that Pneumocystis carinii exists as separate mating types, and expresses and regulates proteins that govern meiosis and progression of the life cycle. This
Philip Vitorino et al.
Nature, 519(7544), 425-430 (2015-03-25)
Cell migration is a stepwise process that coordinates multiple molecular machineries. Using in vitro angiogenesis screens with short interfering RNA and chemical inhibitors, we define here a MAP4K4-moesin-talin-β1-integrin molecular pathway that promotes efficient plasma membrane retraction during endothelial cell migration.
Yun Wei et al.
Journal of materials chemistry. B, 1(15), 2066-2071 (2013-04-21)
The imidazolium cation was chosen as protein selective affinity group and a kind of ionic liquid magnetic microspheres was developed by immobilizing imidazolium cations onto the surface of silica-coated magnetic microspheres to form imidazolium ionic liquid modified magnetic microspheres (Fe3O4@SiO2@IL).
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