跳转至内容
Merck
所有图片(1)

Key Documents

GERPN2232

ECL Prime Western Blotting System

Cytiva RPN2232

别名:

Amersham ECL Prime, Chemiluminescent Western blotting kit, chemiluminescent detection reagent

登录查看公司和协议定价


About This Item

分類程式碼代碼:
41105332
NACRES:
NA.32

包裝

pkg of 1 kit

製造商/商標名

Cytiva RPN2232

顏色

Detection reagent 1: Colorless Detection reagent 2: Colorless

一般說明

Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.

Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.

應用

Sensitive quantitative or confirmatory Western Blotting

特點和優勢

  • High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
  • Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
  • Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm.
  • Compatible with Rainbow Molecular Weight Markers and Amersham ECL DualVue Western Blotting Markers.
  • Optimized for use with Amersham Hybond-P (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.
  • Recommended membrane Hybond-P or Hybon ECL

成分

The following components are included in the Amersham ECL Prime detection reagent kit.
  • RPN2232 Solution A: Luminol solution, 50 ml Solution B: Peroxide solution, 50 ml sufficient for 1000 cm2 membrane

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

Color:
Detection reagent 1: colorless
Detection reagent 2: colorless

Sensitivity: ~1 pg

法律資訊

ECL Prime
This product or portions thereof is manufactured and sold under license from Cyanagen Srl and is subject of US patent 7,855,287, US Patent 7,803,573, and Italian application number TO2010A000580, together with other equivalent granted patents and patent applications in other countries.
Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Rainbow is a trademark of Cytiva

相關產品

产品编号
说明
价格

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Cody J Warren et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(23), 11460-11469 (2019-05-23)
Pandemic HIV-1 (group M) emerged following the cross-species transmission of a simian immunodeficiency virus from chimpanzees (SIVcpz) to humans. Primate lentiviruses (HIV/SIV) require the T cell receptor CD4 to enter into target cells. By surveying the sequence and function of
Yana van der Weegen et al.
Nature communications, 11(1), 2104-2104 (2020-05-02)
The response to DNA damage-stalled RNA polymerase II (RNAPIIo) involves the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. The function of the TCR proteins CSB, CSA and UVSSA and the manner in which the core DNA

商品

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

实验方案

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门