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包裝
pack of 10 × 1 mL
製造商／商標名
Cytiva 28-9852-55
儲存條件
(20% Ethanol)
床體積
1 mL
容量
~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)
儲存溫度
2-8°C
相关类别
一般說明
Protein G GraviTrap™ includes ten ready-to-use columns prepacked with 1 mL of Protein G Sepharose™ 4 Fast Flow.
Protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
Protein G GraviTrap™ are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.
應用
Fast and efficient manual purification of monoclonal and polyclonal antibodies
特點和優勢
- Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
- Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
- High purity and recovery with purity >95% in one step and recovery typically 70% to 80%.
- High binding capacity such as Protein G GraviTrap™ ~ 20 mg human IgG/column
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
Daniel Neumeier et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(18), e2113766119-e2113766119 (2022-04-30)
The capacity of humoral B cell-mediated immunity to effectively respond to and protect against pathogenic infections is largely driven by the presence of a diverse repertoire of polyclonal antibodies in the serum, which are produced by plasma cells (PCs). Recent
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
实验方案
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.
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