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重組細胞
expressed in E. coli
形狀
aqueous ethanol suspension
分析物化學類別
proteins (Immunoglobulins of various mammalian species)
標籤範圍
~2 mg per mL
技術
affinity chromatography: suitable
基質
Sepharose 4B Fast Flow
基質活化
cyanogen bromide
基質結合
amino
基質墊片
1 atom
儲存溫度
2-8°C
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一般說明
蛋白G是从G组链球菌菌株G-148中分离得到的细菌胞壁蛋白，它能与免疫球蛋白（IgG）结合。通过木瓜蛋白酶消化从细胞中提取该蛋白，并依次使用DEAE-Sephadex离子交换层析、琼脂糖凝胶偶联人IgG亲和层析以及Sephadex G-200凝胶层析进行纯化。蛋白G与各种多克隆和单克隆IgG结合的pH条件基本在2.8-10之间，在pH 4-5时结合最强，在pH 10时结合最弱。它是强大的IgG检测试剂。
蛋白G是从G组链球菌菌株G-148中分离得到的细菌胞壁蛋白，它能与免疫球蛋白（IgG）结合。通过木瓜蛋白酶消化从细胞中提取该蛋白，并依次使用DEAE-Sephadex离子交换层析、琼脂糖凝胶偶联人IgG亲和层析以及Sephadex G-200凝胶层析进行纯化。蛋白G与各种多克隆和单克隆IgG结合的pH条件基本在2.8-10之间，在pH 4-5时结合最强，在pH 10时结合最弱。它是强大的IgG检测试剂。
P3296-5Ml的最新产品编号为GE17-0618-01
P3296-5Ml的最新产品编号为GE17-0618-01
應用
蛋白G琼脂糖凝胶™ 被用于亲和层析，蛋白质层析，抗体纯化和表征，免疫亲和基质，蛋白A、G和L树脂，蛋白质相互作用以及纯化和检测。蛋白G琼脂糖凝胶™ 已被用于开发一种可确认人血清中是否存在抗促红细胞生成素中和抗体的方法，以及比较马血清中白蛋白和IgG消耗的方法。
外觀
悬浮于20%乙醇中
準備報告
使用重组链球菌蛋白G制备，其中白蛋白结合区已被移除
法律資訊
Sepharose is a trademark of Cytiva
訊號詞
Warning
危險聲明
危險分類
Flam. Liq. 3
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
115.0 °F - closed cup
閃點（°C）
46.1 °C - closed cup
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G Yang et al.
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Journal of immunology (Baltimore, Md. : 1950), 135(4), 2589-2592 (1985-10-01)
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We covalently attached human immunodeficiency virus type 1 (HIV-1) Env SOSIP trimers to iron oxide nanoparticles (IO-NPs) to create a particulate immunogen for neutralizing antibody (NAb) induction. The attached trimers, ∼20 per particle, retained native-like antigenicity, judged by reactivity with
实验方案
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
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