GE17-5280-01

nProtein A Sepharose^™ 4 Fast Flow

Cytiva 17-5280-01, pack of 5 mL

• 别名: Sepharose 4 Fast Flow
• 分類程式碼代碼: 41106500
• NACRES: NA.56

属性

• ligand: native protein A (S. aureus)
• 包裝: pack of 5 mL
• 製造商／商標名: Cytiva 17-5280-01
• 儲存條件: (20% Ehtanol)
• 基質: 4% cross-linked agarose
• 平均直徑: 90 μm (d50v)
• cleaning: 2-10
• 工作範圍: 3-9
• 容量: >30 mg binding capacity(human IgG/ml)
• 適合性: suitable for bioprocess medium
• 儲存溫度: 2-8°C

说明

一般說明

nProtein A Sepharose^™ 4 Fast Flow is native protein A coupled to Sepharose^™ 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose^™ 4 Fast Flow is native protein A coupled to the well established Sepharose^™ 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose^™ 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose^™ 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose^™ CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose^™ 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

As member of the BioProcess media range, nProtein A Sepharose^™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特點和優勢

• Replaces Protein A Sepharose^™ 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
• Used in routine commercial production of monoclonal antibodies
• Free from animal-derived components.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

安全信息

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids