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GE17-5079-02

HiTrap® rProtein A Fast Flow

Cytiva 17-5079-02, pack of 2 × 1 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

包裝

pack of 2 × 1 mL

製造商／商標名

Cytiva 17-5079-02

儲存條件

(20% Ehtanol)
 (20% Ethanol)

參數

<4 mL/min flow rate
42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

4% cross-linked agarose

粒徑

60-165 μm

平均直徑

90 μm (d50v)

cleaning

2-11

工作範圍

3-10

容量

~50 mg binding capacity (human lgG/ml medium)

適合性

suitable for bioprocess medium

儲存溫度

2-8°C

相关类别

一般說明

Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.
HiTrap® rProtein A FF columns are prepacked with rProtein A Sepharose™ Fast FLow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

應用

The recombinant Protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast FLow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

特點和優勢

  • Quick, convenient preparative purification of monoclonal antibodies.
  • Well-established Protein A-based medium used in many approved MAb processes.
  • Recombinant Protein A exhibits similar Fc region specificity to that of native Protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA® design.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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商品

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

实验方案

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

线性流速（cm/小时）与体积流速（mL/min)之间的换算

本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

亲和层析缓冲液置换和脱盐

如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。

查看所有结果

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