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ligand
recombinant protein A (E. coli)
包装
pack of 25 mL
制造商/商品名称
Cytiva 17-1279-02
储存条件
(20% Ehtanol)
基质
4% cross-linked agarose
粒径
60-165 μm
平均直径
90 μm (d50v)
cleaning in place
2-11
工作范围
3-10
容量
~50 mg binding capacity (human lgG/ml medium)
适用性
suitable for bioprocess medium
储存温度
2-8°C
一般描述
rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale.
Monoclonal and polyclonal antibody purification
rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.
Protein A resin characteristics
The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.
Monoclonal and polyclonal antibody purification
rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.
Protein A resin characteristics
The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.
特点和优势
- For monoclonal and polyclonal antibody purification at both laboratory and process scale.
- Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes.
- Free from animal-derived components.
- Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
实验方案
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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