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GE11-0034-95

HiTrap® MabSelect SuRe™

Cytiva 11-0034-95, pack of 5 × 5 mL

别名:

prepacked columns, MabSelecT columns

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

alkali-stabilized protein A-derived (E. coli)

包裝

pack of 5 × 5 mL

製造商／商標名

Cytiva 11-0034-95

儲存條件

(20% Ehtanol)
 (20% Ethanol)

參數

flow rate
42 psi

床尺寸

16 mm × 25 mm

床體積

5 mL

柱內徑

16 mm

基質

highly cross-linked agarose

樣品平均尺寸

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-13.7

工作範圍

3-12

容量

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

適合性

suitable for bioprocess medium

儲存溫度

2-8°C

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相关类别

一般說明

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect™ SuRe
HiTrap® MabSelect™ SuRe™ columns are prepacked with MabSelect™ SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).

HiTrap® MabSelect™ columns are prepacked with MabSelect™ a bioprocess medium for capuring of Mabs from large sample volumes.

特點和優勢

  • Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
  • Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva
MabSelect SuRe is a trademark of Cytiva
SuRe is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

实验方案

Performing a Purification of IgG Antibodies with MabSelect Sure

This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from Cytiva.

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