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G8404

Sigma-Aldrich

葡萄糖-6-磷酸脱氢酶 来源于肠系膜明串珠菌

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

别名:

Entner-Doudoroff酶, G6PD, G6PDH, NADP 葡萄糖 6-磷酸脱氢酶, G-6-P-DH

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About This Item

CAS号:
9001-40-5
EC 号:
1.1.1.49 (BRENDA, IUBMB)
MDL號碼:
MFCD00081656
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Leuconostoc mesenteroides)

品質等級

300

重組細胞

expressed in E. coli

形狀

ammonium sulfate suspension

比活性

≥550 units/mg protein (biuret)

分子量

128 kDa

儲存條件

(Tightly closed)

技術

cell culture | mammalian: suitable

UniProt登錄號

UPI0021A4D550

異物活動

creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%

儲存溫度

2-8°C

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相关类别

一般說明

葡萄糖6-磷酸脱氢酶（G-6-P-DH）是磷酸戊糖途径第一步的关键调节酶。G-6-P-DH是一种糖蛋白，分子量为 128 kDa（凝胶过滤）。G6PD 是二聚体，每个亚基上有一个活性位点。

研究领域：细胞信号传导

應用

葡萄糖-6-磷酸脱氢酶已用于：

  • 测试玉米胚乳发育过程中的酮糖还原酶活性。
  • 测定冠心病患者血清中甘露糖的水平
  • 研究其对细胞外聚合物 (EPS) 提取物的反应活性，超声法测定细胞裂解液
  • 测定培养人体肌肉卫星细胞的葡萄糖摄入情况

生化／生理作用

G6PD可为所有细胞提供还原动力（如烟酰胺腺嘌呤二核苷酸磷酸 (NADPH)），使细胞能够平衡氧化压力。G6PD基因突变与 X 连锁G6PD缺陷有关——这是一种遗传性基因缺陷，包括新生儿黄疸和急性溶血性贫血等。
葡萄糖-6-磷酸脱氢酶(G6PD)催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯，作为磷酸戊糖途径的第一步。

單位定義

在 NAD 存在下，在pH 7.8，30°C 下，1 个单位将每分钟氧化 1.0 μmol D-6-磷酸葡萄糖至 6-磷酸-D-葡萄糖酸盐。

外觀

用 3.2 M (NH 4 ) 2 SO 4 （含 50 mM Tris 和 1 mM MgCl 2 ，pH 7.5）混悬

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Angelo Minucci et al.
IUBMB life, 61(1), 27-34 (2008-10-23)
Glucose 6-phosphate dehydrogenase (G6PD) deficiency is the most common defect of red blood cells. Although some different laboratory techniques or methods are employed for the biochemical screening, a strict relationship between biochemists, clinicians, and molecular biologists is necessary for a
Charles-David Dubé et al.
AMB Express, 9(1), 23-23 (2019-02-08)
Extracellular polymeric substances (EPS) play major roles in the efficacy of biofilms such as anaerobic granules, ranging from structural stability to more specific functions. The EPS of three granular anaerobic sludges of different origins were studied and compared. Particularly, the
Hemang M Parikh et al.
BMC endocrine disorders, 21(1), 32-32 (2021-03-01)
Insulin resistance (IR) in skeletal muscle is a key feature of the pre-diabetic state, hypertension, dyslipidemia, cardiovascular diseases and also predicts type 2 diabetes. However, the underlying molecular mechanisms are still poorly understood. To explore these mechanisms, we related global
M D Cappellini et al.
Lancet (London, England), 371(9606), 64-74 (2008-01-08)
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect, being present in more than 400 million people worldwide. The global distribution of this disorder is remarkably similar to that of malaria, lending support to the so-called malaria protection
Mareike Hauenstein et al.
Bio-protocol, 7(18), e2561-e2561 (2017-09-20)
Hydroxylation of chlorophyll catabolites at the so-called C32 position ( Hauenstein et al., 2016 ) is commonly found in all plant species analyzed to date. Here we describe an in vitro hydroxylation assay using Capsicum annuum chromoplast membranes as a
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以硫酸铵悬浮液形式提供的酶的使用指南

实验方案

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

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