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生物源
mouse
品質等級
重組細胞
expressed in E. coli
化驗
>97% (SDS-PAGE)
形狀
lyophilized powder
效力
10-60 pg/mL ED50
品質
endotoxin tested
分子量
19 kDa (179 amino acids including N-terminal methionine)
包裝
pkg of 5 and 25 μg
儲存條件
avoid repeated freeze/thaw cycles
技術
cell culture | mammalian: suitable
雜質
≤0.01 EU/μg
UniProt登錄號
儲存溫度
−20°C
基因資訊
mouse ... Csf3(12985) , Csf3r(12986)
一般說明
The Csf3r (colony stimulating factor 3 receptor (granulocyte)) gene encodes a prototypical mobilizing cytokine.
應用
Granulocyte Colony-Stimulating Factor from mouse has been used in cytokine therapy.
生化／生理作用
G-CSF is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.
Granulocyte colony-stimulating factor (G-CSF) is a lineage-restricted hematopoietic growth factor, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors. G-CSF3,4 is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and mature granulocytes, and promotes differentiation of certain myeloid leukemic cells. Natural G-CSF is a glycoprotein of 177 amino acids and a molecular weight of 19 kDa.5 Human and murine G-CSF have about 75% homology and show biological cross-reactivity.
外觀
Lyophilized from a 0.2 μm filtered solution in 10 mM citric acid, 0.01% TWEEN® 20, pH 4.0, containing 50 μg bovine serum albumin per 1 μg of cytokine.
分析報告
The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
法律資訊
TWEEN is a registered trademark of Croda International PLC
相關產品
产品编号
说明
价格
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Expression of the G-CSF receptor in monocytic cells is sufficient to mediate hematopoietic progenitor mobilization by G-CSF in mice
Christopher MJ, et al.
The Journal of Experimental Medicine (2011)
Hitesh S Deshmukh et al.
Nature medicine, 20(5), 524-530 (2014-04-22)
Neonatal colonization by microbes, which begins immediately after birth, is influenced by gestational age and the mother's microbiota and is modified by exposure to antibiotics. In neonates, prolonged duration of antibiotic therapy is associated with increased risk of late-onset sepsis
Combining pharmacological mobilization with intramyocardial delivery of bone marrow cells over-expressing VEGF is more effective for cardiac repair
Wang Y, et al.
Journal of Molecular and Cellular Cardiology, 40(5), 736-745 (2006)
Expression of the G-CSF receptor in monocytic cells is sufficient to mediate hematopoietic progenitor mobilization by G-CSF in mice
ChristopherMJ, et al.
The Journal of Experimental Medicine (2011)
Rui Zhang et al.
Hematology (Amsterdam, Netherlands), 20(10), 555-560 (2015-06-13)
The treatment for acute myeloid leukemia (AML) remains an important clinical problem. Recently, hematopoietic stem cell therapy provides promising outcomes. In this study we examined the clinical effect of HLA-mismatched hematopoietic stem cell infusion combined with chemotherapy as a postremission
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