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品質等級
技術
affinity chromatography: suitable
基質活性組
phase
膨潤
1 g swells to 9-11 mL
珠尺寸
20-80 μm
應用
life science and biopharma
相容性
Cytiva
一般說明
Sephadex G-50(细颗粒)葡聚糖凝胶是广泛使用的凝胶过滤树脂,设计用于分子量30,000以上生物分子的脱盐和缓冲液置换。因其胶珠较小而具有高效率。不同类型的Sephadex的交联度不同,因此其溶胀程度和分子分离范围也不同。Sephadex G-50属于G型,G型Sephadex的型号包括从小分子G-10到大分子G-75的五种型号。Sephadex G-50分为4种不同粒径,包括粗(Coarse)、中(Medium)、细(Fine)和超细(Superfine)颗粒。
Sephadex® G-50是一种用于亲和层析、蛋白质层析和凝胶过滤层析的凝胶过滤介质。Sephadex 是一种由葡聚糖和环氧氯丙烷交联而成的珠状凝胶。
分馏范围(MW)
球状蛋白质:1,500-30,000
葡聚糖:500-10,000
分馏范围(MW)
球状蛋白质:1,500-30,000
葡聚糖:500-10,000
應用
Sephadex® G-50 适用于:
- 低分子量和高分子量分子的分离
- 亲和色谱法、蛋白质色谱法和凝胶过滤色谱法
Sephadex® G-50葡聚糖凝胶适于:
- 去除脂质体悬液中的非包埋羧基荧光素(CF)
- 通过离心式尺寸排阻色谱纯化人MN-14单克隆抗体(mAb)
- 对5和6部分洗脱的重组蛋白进行脱盐
特點和優勢
- 一步完成脱盐、除污染和置换新缓冲液。
- 适于通过凝胶过滤纯化DNA,除去小分子。
- 凝胶珠较小,扩散距离更短。
- 经典的凝胶过滤树脂。
- Sephadex 脱盐被认为优于透析,因为它节省时间,稀释系数低,即便使用少量样品也能恢复高活性
其他說明
G5080-100G′更新后的产品编号为 GE17-0042-01
G5080-500G′更新后的产品编号为 GE17-0042-02
G5080-500G′更新后的产品编号为 GE17-0042-02
法律資訊
Sephadex is a registered trademark of Cytiva
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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