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品質等級
技術
buffer exchange: suitable
基質活性組
phase
膨潤
1 g swells to 4-6 mL
珠尺寸
50-150 μm
應用
life science and biopharma
相容性
Cytiva
InChI
1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
InChI 密鑰
BCPVZFFJGCURNR-OCOFDJSDSA-N
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一般說明
Sephadex® G-25葡聚糖凝胶由葡聚糖与环氧氯丙烷交联而成,用作体积排阻和凝胶过滤树脂。不同类型的Sephadex的交联度不同,因此其溶胀程度和分子分离范围也不同。Sephadex G-25是G型Sephadex中的一种型号。G型系列分5种型号,具有不同的分子量分离范围。其中,G-10适合分离小分子,G-75适合大分子。Sephadex G-25保证可靠供应并提供全面的技术和监管支持,以满足工业要求。
應用
Sephadex® G-25葡聚糖凝胶可用于通过凝胶渗透色谱将标记蛋白与未反应染料N-羟基琥珀酰亚胺酯(NHS)分离。
分馏范围(MW)
右旋糖酐:100 - 5,000
球状蛋白质1,000 - 5,000
Sephadex® G-25已用于:
右旋糖酐:100 - 5,000
球状蛋白质1,000 - 5,000
Sephadex® G-25已用于:
- 蛋白酶脱盐
- 在放射性碘标记oTP-1的表征和受体分析确认过程中,用色谱柱从游离碘中分离放射性碘标记的oTP-1
生化/生理作用
Sephadex® G-25葡聚糖凝胶是应用广泛的凝胶过滤树脂,主要用于工业应用中的脱盐和缓冲液置换。亲水基质可最大限度减少非特异性吸附,获得高收率的蛋白和核酸。Sephadex® G-25常用于亲和层析、蛋白质层析和凝胶过滤层析。
特點和優勢
- 一步完成快速脱盐、除污染和置换新缓冲液。
- 高回收率,极低的样品稀释度。
- 提供预装树脂的HiPrep脱盐柱和HiTrap脱盐柱,用于方便快速地脱盐
- 专门设计用作生物工艺树脂,适合工业应用。
法律資訊
Sephadex is a registered trademark of Cytiva
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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