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质量水平
表单
(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)
分析物化学类别
proteins (GST)
包装
set of 3 pre-packed columns (2.5 ml each)
技术
immunoprecipitation (IP): suitable
protein purification: suitable
基质
cross-linked 4% beaded agarose
储存温度
2-8°C
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一般描述
这些柱中的树脂由通过硫连接至环氧活化的4%交联琼脂糖珠上的谷胱甘肽组成，形成12个原子间隔基。
应用
利用谷胱甘肽-琼脂糖的亲和层析技术可以快速、温和、非变性和高度选择性地纯化谷胱甘肽结合酶，如谷胱甘肽-S-转移酶、谷胱甘肽过氧化物酶和乙二醛酶I。
该产品用于新型色胺和四氢-β-咔啉基CDK4选择性抑制剂的设计、合成和生物学评价。它还可用于研究Fascaplysin激发的二吲哚作为CDK4/细胞周期素D1的选择性抑制剂。
该产品用于新型色胺和四氢-β-咔啉基CDK4选择性抑制剂的设计、合成和生物学评价。它还可用于研究Fascaplysin激发的二吲哚作为CDK4/细胞周期素D1的选择性抑制剂。
利用谷胱甘肽-琼脂糖亲和层析技术，可以快速、温和、非变性和高度选择性地纯化含有谷胱甘肽结合序列的蛋白质，如谷胱甘肽S-转移酶(GST)、谷胱甘肽过氧化物酶和乙二醛酶I。
联系
产品悬浮液编号G 4510
外形
0.5 M NaCl + 20%乙醇溶液的混悬液（1:1）
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
180.1 °F
闪点(°C)
82.3 °C
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F Toribio et al.
Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography
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We present the design, synthesis and biological activity of a library of substituted (biphenylcarbonyl)-tryptamine and (biphenylcarbonyl)-tetrahydro-beta-carboline compounds related to the natural product fascaplysin, as novel inhibitors of CDK4/cyclin D1. We show all these molecules, prepared using the Suzuki-Miyaura reaction, being
Diane M Kanter et al.
Nucleic acids research, 39(7), 2580-2592 (2010-11-27)
Sld2 is essential for the initiation of DNA replication, but the mechanism underlying its role in replication is not fully understood. The S-phase cyclin dependent kinase (S-CDK) triggers the association of Sld2 with Dpb11, and a phosphomimetic mutation of Sld2
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Organic & biomolecular chemistry, 4(5), 787-801 (2006-02-24)
We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound
Xiu Feng Hu et al.
The Journal of clinical investigation, 119(2), 362-375 (2009-01-17)
Provirus integration site for Moloney murine leukemia virus (PIM1) is a proto-oncogene that encodes a serine/threonine kinase with multiple cellular functions. Overexpression of PIM-1 plays a critical role in progression of prostatic and hematopoietic malignancies. Here we describe the generation
相关内容
利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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