G211050

DMT-2′O-TBDMS-rG（ib）亚磷酰胺

configured for (ÄKTA^® and OligoPilot^®)

• 别名: N-（2-甲基-1-氧代丙基）-5′-O- [双（4-甲氧基苯基）苯基甲基]-2′-O-[（1,1-二甲基乙基）二甲基甲硅烷基]-鸟苷，3′-[2-氰基乙基]，N-双（1-甲基乙基）亚磷酰胺]
• 经验公式（希尔记法）: C₅₀H₆₈N₇O₉PSi
• CAS号: 147201-04-5
• 分子量: 970.18
• MDL號碼: MFCD00080299
• 分類程式碼代碼: 12352200
• PubChem物質ID: 329799857
• NACRES: NA.51

属性

• 生物源: non-animal source (no BSE/TSE risk)
• 品質等級: 300
• 產品線: Proligo Reagents
• 化驗: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• 形狀: powder
• 分子量: 970.18 g/mol
• 技術: oligo synthesis: suitable
• 雜質: ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤0.5% single Impurity (reversed phase HPLC); ≤3% residual Solvent content
• 顏色: white to off-white
• &lambda ；: conforms (UV/VIS Identity)
• 適合性: conforms to structure for H-NMR; conforms to structure for LC-MS
• 相容性: configured for (ÄKTA^® and OligoPilot^®)
• 核苷譜: base: guanosine; base protecting group: isobutyryl; 2' protecting group: TBDMS; 5' protecting group: DMT; deprotection: standard
• 儲存溫度: −20°C
• SMILES 字串: COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](O[Si](C)(C)C(C)(C)C)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(NC(=O)C(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C50H68N7O9PSi/c1-32(2)45(58)54-48-53-44-41(46(59)55-48)52-31-56(44)47-43(66-68(12,13)49(7,8)9)42(65-67(63-29-17-28-51)57(33(3)4)34(5)6)40(64-47)30-62-50(35-18-15-14-16-19-35,36-20-24-38(60-10)25-21-36)37-22-26-39(61-11)27-23-37/h14-16,18-27,31-34,40,42-43,47H,17,29-30H2,1-13H3,(H2,53,54,55,58,59)/t40-,42-,43-,47-,67?/m1/s1
• InChI 密鑰: PGJKESPHANLWME-FTZVBZPOSA-N

说明

一般說明

DMT-2′O-TBDMS-rG(ib) Phosphoramidite belongs to the group of 2′O-TBDMS RNA Phosphoramidites.

應用

RNA interference (RNAi) has become a popular tool for the sequence-specific inhibition of gene expression and can be used in target validation and other drug development techniques. The most convenient method to provide sequence-specific RNA oligonucleotides is a chemical synthesis on a solid support with RNA phosphoramidites and RNA CPG, analogous to DNA synthesis.

特點和優勢

• Industry-standard 2′O-TBDMS protective group
• Consistent lot-to-lot purity and performance
• Compatible with deprotection methods based on methylamine or AMA
• Standard RNA phosphoramidites provide excellent coupling results when used with ETT or BTT as an activator; best results are obtained with Activator 42
• Capping with standard acetic anhydride capping reagent rather than with Fast Deprotection Cap A
• Manufactured under a certified ISO 9001 quality system

其他說明

The unique properties of RNA have stimulated the development of a variety of applications in diagnostics and therapeutics, as well as in basic molecular biology research where RNA can be used as:

• Catalytic agents (ribozymes)
• Affinity ligands (aptamers)
• Agents to induce gene silencing (RNA interference) (a)

法律資訊

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable