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生物来源
mouse
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
FKH 117, monoclonal
表单
buffered aqueous solution
分子量
antigen 70-75 kDa
种属反应性
human
技术
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total extract of COS-7 cells transfected with FKHR expression plasmid
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... FOXO1(2308)
一般描述
FKHR belongs to the superfamily of forkhead transcription factors (FOX). It is characterized by a common Forkhead (or winged helix) domain, a variant of the helix-turn-helix motif. FKHR contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB).
Monoclonal Anti-FKHR (FOXO1a) (mouse IgG1 isotype) is derived from the FKH117 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human FKHR, conjugated to KLH.
免疫原
synthetic peptide corresponding to human FKHR (amino acids 636-651), conjugated to KLH.
应用
Monoclonal Anti-FKHR (FOXO1a) antibody is suitable for immunoblot assay to study the down-regulation of both p16INK4A and p14ARF expression, and immunoblot of SKMCs and HSMM cells in a study of stable ectopic expression of PAX3-FKHR in low passage primary human skeletal muscle cells. It is also suitable for microarray and indirect ELISA.
Monoclonal Anti-FKHR antibody has been used in:
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
生化/生理作用
FKHR plays a key regulatory roles in embryonic development, differentiation, apoptosis, and tumorigenesis. It was first identified at chromosomal breakpoints in human tumors and consequently linked to tumorigenesis. The activity of FKHR is regulated by growth factors via the PKB/PI3K pathway. The expression of FKHR is inhibited through phosphorylation by PKB. Within the nucleus, the dephosphorylated FKHRL1 induces expression of target genes such as Fas ligand, and triggers apoptosis. It is involved in apoptosis, glucose homeostasis, cell cycle regulation and as a nuclear receptor co factor. It has been found that FKHR also play a role in endometrial differentiation via the protein kinase A (PKA) pathway.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
A Mansouri
Critical reviews in oncogenesis, 9(2), 141-149 (1999-02-11)
Pax genes consist of a family of transcription factors that are essentially required for the genesis of a variety of tissues and organs. Pax3 and Pax7 are specifically expressed in the dorsal neural tube and the developing somite. Loss-of-function mutations
Mark Christian et al.
The Journal of biological chemistry, 277(23), 20825-20832 (2002-03-15)
Decidual transformation of human endometrial stromal (ES) cells requires sustained activation of the protein kinase A (PKA) pathway. In a search for novel transcriptional mediators of this process, we used differential display PCR analysis of undifferentiated primary ES cells and
FGFR4 Blockade Exerts Distinct Antitumorigenic Effects in Human Embryonal versus Alveolar Rhabdomyosarcoma
Crose LES, et al.
Clinical Cancer Research, 18(14), 3780-3790 (2012)
The PAX3-FKHR Fusion Gene of Rhabdomyosarcoma Cooperates with Loss of p16INK4A to Promote Bypass of Cellular Senescence
Linardic CM, et al.
Cancer Research, 67(14), 6691-6699 (2007)
Defining the Cooperative Genetic Changes That Temporally Drive Alveolar Rhabdomyosarcoma
Naini S, et al.
Cancer Research, 68(23), 9583-9588 (2008)
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