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Merck
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文件

E7637

Sigma-Aldrich

溴化乙锭

BioReagent, for molecular biology, powder

别名:

3,8-二氨基-5-乙基-6-苯基菲啶溴化物, EtBr, 胡米溴铵

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About This Item

经验公式（希尔记法）:
C21H20BrN3
CAS号:
1239-45-8
分子量:
394.31
Beilstein:
3642536
EC號碼:
214-984-6
MDL號碼:
MFCD00011724
分類程式碼代碼:
41105322
PubChem物質ID:
24894653
NACRES:
NA.31

等級

for molecular biology

品質等級

200

產品線

BioReagent

形狀

powder

技術

electrophoresis: suitable

mp

260-262 °C (dec.) (lit.)

螢光

λex 360 nm; λem 590 nm in PBS
λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;)
λex 526 nm; λem 605 nm in aqueous buffer

適合性

suitable for gel electrophoresis

SMILES 字串

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI 密鑰

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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應用

溴化乙锭 (EtBr) 是 PAGE 或琼脂糖凝胶电泳最常用的核酸染色剂。EtBr 的荧光在与双链 RNA 结合后可增加 21 倍，在与双链 DNA 结合后增加 25 倍，因此在低染色浓度（10μg/ml）下无需本底脱色。溴化乙锭已用于大量核酸荧光测定。 它已被证明可与单链 DNA（虽然没有那么强）和三链 DNA 结合。由于它能与 DNA 结合，EtBr 是 DNA 聚合酶的抑制剂。

插入双链 DNA 和 RNA 的移码诱变剂。

生化／生理作用

溴化乙啶可插入双链 DNA 和 RNA 中，并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。

重構

对于在电泳后进行凝胶染色，应先用水将储存液样品稀释到 0.5μg/ml，再将凝胶孵育 15-30 分钟。通常无需脱色，但是如果必须降低背景色，则可在水中脱色 15 分钟。然后可在紫外灯箱（254nm 波长）中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液（终浓度 0.5μg/ml）中，并在电泳后立即显影。

相關產品

产品编号
说明
价格

象形圖

Skull and crossbonesHealth hazard
GHS06,GHS08

訊號詞

Danger

危險聲明

H302,H330,H341

危險分類

Acute Tox. 1 Inhalation - Acute Tox. 4 Oral - Muta. 2

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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