推荐产品
生物来源
bacterial (Leuconostoc mesenteroides)
质量水平
表单
lyophilized powder
比活
≥100 units/mg protein
组成
Protein, ~15% Lowry
溶解性
H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
储存温度
−20°C
一般描述
Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.
应用
Dextran Sucrase from Leuconostoc mesenteroides has been used:
- in immobilization on shirasu porous membrane (SPG) for dextran production
- in the enzymatic synthesis of dextran nanoparticles at various pH range
- in the immobilization with hydroxyapatite for dextran production
Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.
The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.
生化/生理作用
Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages. The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad. The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.
质量
色谱纯化产品
单位定义
One unit will liberate 1.0 μmole of fructose per min at 37 °C, pH 5.4.
外形
Lyophilized powder containing dextran, MES buffer salts and CaCl2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Seo Eun Chae et al.
Journal of microbiology and biotechnology, 19(12), 1644-1649 (2010-01-16)
This study investigates the buffering effects of calcium salts in kimchi on total acidity, microbial population, and dextransucrase activity. Calcium chloride or calcium carbonate was added in dongchimi-kimchi, a watery-radish kimchi, and their effects on various biochemical attributes were analyzed.
Sophie Bozonnet et al.
Journal of bacteriology, 184(20), 5753-5761 (2002-09-25)
A novel Leuconostoc mesenteroides NRRL B-1299 dextransucrase gene, dsrE, was isolated, sequenced, and cloned in Escherichia coli, and the recombinant enzyme was shown to be an original glucansucrase which catalyses the synthesis of alpha-1,6 and alpha-1,2 linkages. The nucleotide sequence
M Kobayashi et al.
Biochimica et biophysica acta, 614(1), 46-62 (1980-07-10)
Multiple forms of dextransucrase (sucrose:1.6-alpha-D-glucan 6-alpha-D-glucosyltransferae EC 2.4.1.5) from Leuconostoc mesenteroides NRRL B-512F strain were shown by gel filtraton and electrophoretic analyses. Two components of enzyme, having different affinities for dextran gel, were separated by a column of Sephadex G-100.
S A Ul-Qader et al.
Biotechnology and applied biochemistry, 34(Pt 2), 93-97 (2001-10-11)
A newly isolated strain of Leuconostoc mesenteroides (PCSIR-3) produced a different dextran compared with that of L. mesenteroides NRRL B-512F. Different media compositions used for dextran production showed that media containing CaCl(2) produced dextran in higher quantities compared with other
Pore control with dextran generated from immobilized dextransucrase
Kawakita H, et al.
Biochemical Engineering Journal, 36(2), 190-193 (2007)
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