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生物来源
bovine spleen
类型
Type V
表单
essentially salt-free, lyophilized powder
比活
≥1,000 units/mg protein
分子量
38 kDa
浓度
≥50% protein
技术
DNA extraction: suitable
tissue culture: suitable
适用性
suitable for molecular biology
应用
cell analysis
异质活性
RNase ≤0.1%
储存温度
−20°C
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应用
牛脾脱氧核糖核酸酶Ⅱ已用于：
- 作为从结肠样品分离免疫细胞的消化混合液组分
- 消化视网膜外植体以制备单细胞悬液
- 分离人结肠组织的基质细胞
- 作为从大鼠心脏制备胚胎心肌细胞的解离培养基组分
生化/生理作用
脱氧核糖核酸酶II，也称为酸性DNA酶，可水解天然和变性DNA中的脱氧核糖核苷酸连接键，产生带有3′-磷酸的产物。体外最佳pH范围是4.5-5.0。它还可在pH 5.6-5.9下作用于对硝基苯基磷酸二酯。分子量约为38,000 Da。
单位定义
在 pH 4.6 25℃条件下，一个Kunitz单位可每分钟在每毫升内产生0.001的ΔA260；[Mg2+] = 0.83 mM
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
K Nitta et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 114(2), 119-128 (1994-02-01)
Deoxyribonucleases from eggs and the liver of Xenopus laevis were partially purified by DEAE-cellulose and heparin-Sepharose affinity column chromatographies. The fractions having egg and liver DNase activities were eluted on high performance liquid chromatography through TSK gel G3000SW at the
Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.
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The Journal of Physiology, 450(1), 409-435 (1992)
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An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
STUDIES ON ACID DEOXYRIBONUCLEASE. II. ISOLATION AND CHARACTERIZATION OF SPLEEN-ACID DEOXYRIBONUCLEASE.
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DNase II is an endonuclease which plays a fundamental role in the degradation of DNA from both apoptotic cells, and nuclei extruded from red blood cells during erythropoiesis: important tasks, considering that everyday 10(8)-10(9) cells undergo apoptosis, and 10(11) red
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