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Merck

D2196

Sigma-Aldrich

DAF-FM solution

5 mM in DMSO, ≥97% (HPLC)

别名:

4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM

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About This Item

经验公式(希尔记法):
C21H14F2N2O5
分子量:
412.34
分類程式碼代碼:
12352200
NACRES:
NA.77

品質等級

化驗

≥97% (HPLC)

形狀

DMSO solution

儲存條件

desiccated

顏色

colorless to yellow, including pink

運輸包裝

wet ice

儲存溫度

−20°C

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

InChI 密鑰

DIJCILWNOLHJCG-UHFFFAOYSA-N

應用

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

生化/生理作用

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

特點和優勢

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Detection of protein S-nitrosothiols (SNOs) in plant samples on diaminofluorescein (DAF) gels
Rodriguez-Ruiz M, et al.
Bio-protocol null
Marta Rodríguez-Ruiz et al.
Bio-protocol, 7(18), e2559-e2559 (2017-09-20)
In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems (

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