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产品线
BioReagent
方案
≥95% (HPLC)
mp
232-234 °C (lit.)
溶解性
DMSO: soluble
荧光
λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
适用性
suitable for fluorescence
储存温度
2-8°C
SMILES字符串
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
InChI key
VQVUBYASAICPFU-UHFFFAOYSA-N
相关类别
一般描述
2′,7′-二氯荧光素二乙酸酯，也称为 DCFHDA 或 Dcfh-DA，是一种敏感的细胞可渗透氧化还原探针。亲脂性非荧光 Dcfh-DA 通过被动扩散和脱乙酰作用很容易穿过细胞膜。脱酰产物是一种对氧化剂敏感的 2′,7′-二氯荧光素（DCHF）。 DCHF 随后被氧化以形成高荧光 DCF，这是被测量的。
应用
2′,7′-二氯荧光素二乙酸酯主要用于检测活性氧（ROS）中间体并量化细胞中的过度 氧化应激 。
Dcfh-DA 用于定量测定，以测量纳米颗粒处理的细胞中的氧化应激。
Dcfh-DA 用于定量测定，以测量纳米颗粒处理的细胞中的氧化应激。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
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