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Merck

D0822

Sigma-Aldrich

DMEM - high glucose

StableCell, with stable glutamine, sodium pyruvate and sodium bicarbonate, sterile liquid, suitable for cell culture

别名:

杜氏改良 Eagle 培养基 - 高葡萄糖, DME, DMEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

StableCell DMEM - 高葡萄糖, With 4500 mg/L glucose, stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

Endotoxin, tested

成分

glucose: high
HEPES: no
stable glutamine
sodium pyruvate: yes
phenol red: yes
NaHCO3: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

该DMEM-Hi葡萄糖培养基与原始配方不同,因为它补充有L-丙氨酰-L-谷氨酰胺二肽。 众所周知,谷氨酰胺在细胞培养中不稳定。该二肽提供更稳定形式的谷氨酰胺用于细胞培养。

應用

Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。

法律資訊

StableCell is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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