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Key Documents

CMC0016

Sigma-Aldrich

BL21(DE3)电感受态细胞

Escherichia coli, rod shaped

别名:

BL21 strain

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About This Item

分類程式碼代碼:
41106202
NACRES:
NA.85

product name

BL21(DE3)电感受态细胞, for protein expression

生物源

Escherichia coli

等級

for molecular biology

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

相关类别

一般說明

BL21（DE3）电转感受态细胞是第一个在相同细胞中实现高效克隆和高水平蛋白质表达的细胞。
克隆效率比其它 BL21 细胞制剂提高了 25-1,000 倍，这对于构建复杂的表达文库至关重要。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

特點和優勢

BL21（DE3）电转感受态细胞（>5 × 109 cfu/μg）前所未有的转化效率消除了将质粒从克隆菌株转移到表达菌株的需要，从而为典型的克隆和表达实验节省了数天的工作时间。

成分

  • BL21（DE3）电转感受态细胞
  • pUC19转化对照DNA
  • 表达恢复培养基


儲存類別代碼

10 - Combustible liquids

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Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
Felix Nicolaus et al.
eLife, 10 (2021-02-09)
We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
Zev A Ripstein et al.
eLife, 9 (2020-01-10)
The ClpXP degradation machine consists of a hexameric AAA+ unfoldase (ClpX) and a pair of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently degrade client proteins. ClpXP is an important target for drug development against infectious diseases. Although
Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows
M'Lynn E Fisher et al.
eLife, 10 (2021-06-03)
The sarco-plasmic reticulum calcium pump (SERCA) plays a critical role in the contraction-relaxation cycle of muscle. In cardiac muscle, SERCA is regulated by the inhibitor phospholamban. A new regulator, dwarf open reading frame (DWORF), has been reported to displace phospholamban
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实验方案

BL21(DE3) Electrocompetent Cells

Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.

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