SK-PN-DW

94092301, human nerve, Polygonal

• 分類程式碼代碼: 41106514

属性

• product name: SK-PN-DW, 94092301
• 生物源: human nerve
• 增長模式: Semi-adherent
• 染色體組型: 82XXY
• 形態學: Polygonal
• 產品: Pro-cholecystokinin (Pro-cck)
• 受體: Vimentin, P53, anti-MIC2
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human primitive neuroectodermal tumour

細胞系描述

SK-PN-DW has been derived from a male patient with a primitive neuroectodermal tumour that expresses cholecystokinin (cck) RNA and synthesises substantial amounts of pro-cck. SK-PN-DW is able to process the pro-hormone, producing immunoreactive cck-like material plus a peptide that immunochemically and chromatographically resembles intact cck c-terminal extension peptide. Cells have a tendency to float.

應用

Tumour lines should prove useful in understanding the regulation of the cck gene in human neuronal cells and will also provide an in vitro system for investigation of the post translational processing of the cck preprohormone.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 13,14
D7S820: 8,14
THO1: 6
TPOX: 8,9
vWA: 16

培養基

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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