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product name
KYSE-270, 94072021
生物源
human esophagus
增長模式
Adherent
染色體組型
Hypotriploid with 16% polyploidy
形態學
Epithelial
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human oesophageal squamous cell carcinoma
細胞系描述
Established from the well differentiated invasive oesophageal squamous cell carcinoma resected from the middle intra-thoracic oesophagus of a 79-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC.
培養基
RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS)
例行更新培養
Split confluent cultures 1:5 every second day using trypsin/EDTA;5% CO2; 37°C. Doubling time: 24 hours. Recommended seeding density: 2.5 x 10,000 cells/cm2.
其他說明
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