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product name
RENT RO3, 92092514
生物源
rat endometrium
增長模式
Adherent
染色體組型
Not specified
形態學
Spindle shaped
產品
Not specified
受體
Glucocorticoid
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed
細胞系描述
Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, uvomorulin, proenkephalin, desmin and connexin 43 markers.
培養基
DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
例行更新培養
Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 35 h.
其他說明
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